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Journal of Environmental Toxicology 2010;25(2):111-120.
국내 디젤 차량 배기 입자가 쥐 대식세포에 미치는 세포독성 평가
이장한, 이용권, 이지영, 이승복, 김선화, 배귀남, 이학성, 임철수, 정남현
1고려대학교 생명공학과
2유한대학 식품영양과
3고려대학교 생명공학과
4한국과학기술연구원 지구환경센터
5한국과학기술연구원 지구환경센터
6한국과학기술연구원 지구환경센터
7서원대학교 환경건설정보학과
8국립환경과학원 교통환경연구소
9고려대학교 생명공학과
Cytotoxicity of Diesel Exhaust Particles from Various Vehicles toward Macrophage Cells
Jang Han Lee , Yong Kwon Lee , Ji-Young Lee , Seung-Bok Lee , Sun-Hwa Kim , Gwi-Nam Bae , Hak-Sung Lee , Cheol-Soo Lim , Namhyun Chung
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ABSTRACT
  DEPs (diesel exhaust particles) like any other particles can be also inhaled into lung to participate in a damaging reaction to the organ. Possible damages might be apoptosis and inflammatory responses to the cells in respiratory track. The aim of this study was cytotoxicity evaluation of DEPs from five in-use diesel vehicles using a murine macrophage cell (RAW 254.7). We found that most DEPs have a considerable cytotoxicity compared to the control and SRM 2975. When measured by MIT assay and extents of apoptosis, DEPs of two high-mileage vehicles had higher toxicity than those of the other three low-mileage vehicles tested. Although mRNA expression level of TNF-α. somewhat explains the trend of cytotoxicity and apoptosis, that of IL-1β did not. Correlation studies among the extents of MIT assay, apoptosis, and TNF-α. expression showed that the extents between apoptosis and TNF-α expression was most highly correlated (r=0.96). These results suggest that cytotoxicity of various DEPs could be compared easily by measuring the extent of apoptosis or TNF-α expression by DEPs.
Keywords: Diesel exhaust particle; Macrophage; Cytotoxicity; Apoptosis; TNF-α expression
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