1Department of Laboratory Medical Sciences, Al-Aqsa University, Gaza, Palestine 2Faculty of Medicine, Al Azhar University Gaza, Palestine 3Mammalian Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, Egypt 4Department of Chemistry, College of Science, King Khalid University, Abha, Saudi Arabia 5Pesticide Formulation Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Dokki, Egypt 6Faculty of Medical Sciences, Israa University Gaza, Gaza Strip, Palestine 7Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt 8Biochemistry Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt
Received: May 1, 2024; Accepted: September 19, 2024.
ABSTRACT
Magnetite nanoparticles have attracted the attention of researchers for biomedical uses, but their impacts on the reproductive system did not report. Here, we have studied the possible attenuation efficiency of quercetin against magnetite nanoparticles-induced apoptosis in ovarian. Forty female rats were divided equally into control, quercetin (100 mg/kg), magnetite nanoparticles (50 mg/kg), and magnetite nanoparticles+quercetin, where all rats received their doses for four weeks. Compared with the control, magnetite nanoparticles significantly reduced the serum hormonal levels (follicle-stimulating hormone, luteinizing hormone, estrogen, and progesterone) along with glutathione and superoxide dismutase in ovarian tissues. Moreover, magnetite nanoparticles markedly increased the ovarian malondialdehyde, and apoptotic gene expressions (Bax and caspase-3), and induced many histopathological changes. Significantly, co-treatment with quercetin markedly alleviated the hormonal profile, antioxidant disturbance, and ovarian apoptotic pathway of magnetite nanoparticles. Furthermore, our docking study revealed that quercetin could act as a caspase-3 inhibitor and allosteric agonist to follicle-stimulating hormone (Met520 and Val53), luteinizing hormone (Met517, Ala589, Ser604, and Lys595), estrogen (Met421, Phe425, and Ala350), and progesterone (Met759 and Met909) receptors. Those records reveal that the antioxidants and antiapoptotic characteristics are acceptable pointers for female infertility defenders of quercetin, especially during nanoparticle exposure.
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