| Home | E-Submission | Sitemap | Contact Us |  
top_img
Environ Anal Health Toxicol > Volume 36:2021 > Article
Environmental Analysis Health and Toxicology 2021;36(3):e2021017-0. doi: https://doi.org/10.5620/eaht.2021017
Detection of aflatoxin producing Aspergillus flavus from animal feed in Karnataka, India.
Pethannan Rajarajan , Katherin Sylvia, Malaiyarasa Pandian Periasamy , Maheswari Subramanian
Department of Microbiology, Indian Academy Degree College-Autonomous, Bangalore, India
Corresponding Author: Maheswari Subramanian ,Email: mahehrp@gmail.com
Received: December 30, 2020;  Accepted: July 20, 2021.
ABSTRACT
Aflatoxins are toxic carcinogenic secondary metabolite produced by Aspergillus flavus and are responsible for contamination in animal feed. The aim of the study was to determine the prevalence of aflatoxin contamination in animal feed in Karnataka state, India. The screening was performed by desiccated coconut agar and quantification of aflatoxin by liquid ammonia vapor test, TLC and ELISA. A total of 29 samples received from different places of Karnataka were analysed for aflatoxin B1. Out of 29 animal feed sample aflatoxin B1 detected in 12 samples representing 41.38% at average concentration of 288.50 μg/kg. Out of 42 isolates screened in animal feed, Aspergillus flavus was found to be in 86.2% and Aspergillus niger was 24.1%. It was observed that out of 42 isolates analyzed from animal feed, aflatoxin B1 was detected in 12 samples. Aflatoxin B1 is the most common contaminant and the method is more sensitive in screening and detection of aflatoxin B1 in the animal feed.
Keywords: Animal feed, Aspergillus flavus, Aflatoxins, Coconut milk agar, Ammonia vapor test, ELISA
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
  E-Mail
Share:      
METRICS
0
Crossref
0
Scopus
287
View
23
Download
Editorial Office
Division of Environmental Science and Ecological Engineering, Korea University
145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea
Tel : +82-32-560-7520   E-mail: envitoxic@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2021 by The Korean Society of Environmental Health and Toxicology & Korea Society for Environmental Analysis.     Developed in M2PI