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Journal of Environmental Toxicology 2010;25(4):295-306.
성별 및 채뇨 시각별 <SUP>1</SUP>H NMR 기반 뇨 대사체 프로파일링 연구
정진영, 황금숙, 박종철, 김동현, 하미나
1한림대학교 고령사회연구소
2한국기초과학지원연구원 서울센터
3한국기초과학지원연구원 서울센터
4한림대학교 사회의학교실
5단국대학교 예방의학교실
H NMR-Based Urinary Metabolic Profiling of Gender and Diurnal Variation in Healthy Korean Subjects
Jin-Young Jeong , Geum-Sook Hwang , Jong-Chul Park , Dong-Hyun Kim , Mina Ha
: This study was undertaken to examine the metabolomic changes due to gender and diurnal variation at sampling time and to identify an appropriate time point for urine sampling in epidemiologic studies using metabolomic profiles.
  Methods : Urine samples were collected twice a day (morning and afternoon) from 20 healthy Korean adults after fasting for 8 hours. The metabolomic assay was investigated using H NMR spectroscopy coupled with the principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The metabolites responsible for differentiation between groups were identified through the loading plot of PLS-DA and quantified using Chenomx NMR Suite with a 600 ㎒ library.
  Results : Metabolites responsible for differentiation in gender and sampling time were creatinine, trimethyl anine oxide (TMAO), hippurate, mannitol, citrate and acetoacetate. Dimethylamine showed difference only as a factor of diurnal time. The level of creatinine was higher in men compared to women, and the levels of citrate, TMAO, hippurate, mannitol, and acetoacetate were higher in women compared to men. The levels of creatinine, TMAO, hippurate, dimethylamine and mannitol were higher in the morning rather than the afternoon while those of citrate and acetoacetate were higher in the afternoon rather than the morning.
  Conclusions : Since urinary metabolomic profiles varied by gender and diurnal cycle, urine sampling should be performed at the same time point for all participants in epidemiologic studies using metabolomic profiles.
Keywords: Diurnal variation; H NMR spectroscopy; Metabolomic profiles; Multivariate statistical analysis; Urine sample
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