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Journal of Environmental Toxicology 2010;25(3):241-251.
붕어 수정란을 이용한 다이옥신의 초기발생단계 독성평가
박용주, 김하룡, 이민지, 이완옥, 이정식, 정규혁, 오승민
1성균관대학교 약학부
2성균관대학교 약학부
3성균관대학교 약학부
4청평 중앙 내수면 연구소
5전남대학교 수산생명의학부
6성균관대학교 약학부
7호서대학교 GLP 센터
Early Life Stage Toxicity for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Crucian Carp(Carassius auratus)
Yong Joo Park , Ha Ryong Kim , Min Jee Lee , Wan-Ok Lee , Jung Sick Lee , Kyu Hyuck Chung , Seung Min Oh
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ABSTRACT
  Crucian carp (Carassius auratus) has been used as the sentinel species for POPs (Persistent Organic Pollutants) monitoring in aquatic environment. However, there is little information for dioxin toxicity and especially, early life stage toxicity in crucian carp have been never carried out. In this study, we investigated several toxic effects for 2,3,7 ,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in fertilized egg obtained by natural fertilization from crucian carp. The embryos at 3 h post- fertilization (hpf) were treated with 0.039, 0.156, 0.625. and 2.5 (㎍/ℓ) TCDD by waterborne exposure for 60 minutes and changed with fresh water 2 times per day. Fertilized eggs started hatching at 51 hpf and TCDD exposed embryo showed decrease of hatching rate in a dose-dependent manner at 75 hpf. Pericardial edema was continuously observed in larvae exposed to TCDD from hatching start time (51 hpf), followed by the onset of mortality. In addition, AhR-related gene, CYP1A was clearly increased by TCDD in a dose dependent manner. These results indicated that fertilized eggs obtained from crucian carp have the TCDD related gene regulation and a distinct TCDD developmental toxicity syndrome by TCDD exposure. Therefore, we suggested that early life stage test in crucian carp could be used as test methods on dioxins toxicity.
Keywords: Carassius auratus; Early life stage; TCDD developmental toxicity; CYP1A
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