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Journal of Environmental Toxicology 2010;25(1):85-94.
장생도라지 (Platycodon grandiflorum)의 항산화 활성 평가
김충현, 정봉용, 정선기, 이철호, 이현선, 김봉희, 김상겸
1충남대학교 약학대학 형질전환 복제돼지 센터
2충남대학교 약학대학 형질전환 복제돼지 센터
3충남대학교 약학대학 형질전환 복제돼지 센터
4한국생명공학연구원 천연물의약 연구센타
5한국생명공학연구원 천연물의약 연구센터
6충남대학교 약학대학 형질전환 복제돼지 센터
7충남대학교 약학대학 형질전환 복제돼지 센터
Evaluation of Antioxidant Activity of Platycodon grandiflorum
Chung Hyeon Kim , Bong Yong Jung , Sun Ki Jung , Chul Ho Lee , Hyun Sun Lee , Bong-Hee Kim , Sang Kyum Kim
  Platycodon grandiflorum, Doraji as Korean name, is one of the most widely used traditional oriental medicine for bronchial diseases and also used as a folk remedy for geriatric diseases and inflammatory diseases. In recent studies, it has been reported that some effect of P. grandijlorum is derived from its antioxidant activity, although there is still a lack of evidence to establish its oxy-radical scavenging activity. In this study, total oxy-radical scavenging capacity (TOSC) assay was used to evaluate antioxidant activity of total extracts (T-PG), polysaccharide fraction (Po-PG), and saponin fraction (Sa-PG) isolated from P. grandiflorum against peroxyl radicals and peroxynitrites. And MTT assay was taken to assess cyto-protective effects of T-PG, Po-PG and Sa-PG in H4IIE cells treated with hydrogen peroxide and tert-butylhydroperoxide. In the TOSC assay, Sa-PG showed strong oxy-radical scavenging capacity compared with T-PG and Po-PG. In cell-based assay, T-PG and Po-PG protected cells from oxidative stress, but Sa-PG did not protect cells because of cytotoxicity of Sa-PG. These results suggest that the saponin components of P. grandiflorum have relatively strong antioxidant capacity and cytotoxicity in rat hepatoma cells.
Keywords: Platycodon grandiflorum; TOSC assay; cytotoxicity; oxidative stress; antioxidant capacity
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