Evaluation of Hepatic Antioxidant Defense Systems in Rats Treated with Tetrabromobisphenol-A

, Sang yoon Lee; , Kang Uk Yun; , Sun Hong Park; , Sun Ki Jung; , Keon Wook Kang; , Tae Cheon Jeong; , Hyung Sik Kim; , Hye Gwang Jeong; , Bong Hee Kim; , Sang Kyum Kim

Published online January 1, 1986

Journal of Environmental Toxicology 2009;24(4):303-309.

Tetrabromobisphenol-A가 처리된 랫드의 간에서 항산화활성 평가

이상윤, 윤강욱, 박선홍, 정선기, 강건욱, 정태천, 김형식, 정혜광, 김봉희, 김상겸

충남대학교 형질전환복제돼지센터

Evaluation of Hepatic Antioxidant Defense Systems in Rats Treated with Tetrabromobisphenol-A

Sang yoon Lee , Kang Uk Yun , Sun Hong Park , Sun Ki Jung , Keon Wook Kang , Tae Cheon Jeong , Hyung Sik Kim , Hye Gwang Jeong , Bong Hee Kim , Sang Kyum Kim

ABSTRACT

Hepatic antioxidant defense systems were examined in rats treated with tetrabromobisphenol-A (TBBPA), a brominated flame retardant, at the doses of 0, 250, 500 and 1,000 ㎎/㎏ for four weeks. Hepatic ratio of glutathione disulfide to glutathione (GSH) and levels of malondialdehyde, oxidative stress markers were not changed in rats treated with TBBPA. Hepatic expression of antioxidant enzymes including GSH peroxdiase-1 (GPX-1)/GSH reductase (GR), alpha-, mu- and pi-class glutathione-S-transferase (GST) and gamma-glutamylcysteine ligase catalytic subunit was determined using immunoblot analysis. Alpha-class GSTs, GPX-1 and GR levels were significantly decreased in rats treated with TBBPA at the dose of 500 or 1,000 ㎎/㎏. These results show that TBBPA results in down-regulation of hepatic expression of antioxidant enzymes related with GSH, suggesting the liver in TBBPA-treated rats may be more sensitive to oxidants.

Keywords: tetrabromobisphenol-A; antioxidant defense system; oxidative stress; liver