Research of Detection Method for Cytotoxic Effects of Environmental Pollutants Using Atomic Force Microscopy (AFM) in HeLa Cells

, Si-won Lee; , Soo il Lee; , Jinhee Choi

Published online January 1, 1986

Journal of Environmental Toxicology 2008;23(1):47-51.

원자현미경 (AFM)을 이용한 환경오염물질에 노출된 HeLa 세포의 표면변화 연구

이시원, 이수일, 최진희

Research of Detection Method for Cytotoxic Effects of Environmental Pollutants Using Atomic Force Microscopy (AFM) in HeLa Cells

Si-won Lee , Soo il Lee , Jinhee Choi

ABSTRACT

The toxicity of environmental pollutants was measured between a image of the surface topography in HeLa cells using atomic force microscopy for the possibility of toxic effect measurement and environmental monitoring. A image of the surface topography by AFM were estimated as toxic endpoints. The surface topography by AFM was observed a change of the cell surface in the environmental pollutants, but the standard of the measurement requires for the dose-effect degree. The overall results indicate that the possibility of measurement using AFM were confirmed a dose-effect degree related toxic effects, but it requres correlation between more various biomarker and AFM"s measurements if the possibility of the toxic effect measurement was established.

Keywords: atomic force microscopy (AFM); environmental pollutants; HeLa cells; toxicity