Indigenous species means a species that is likely, due to historical presence, to occur at a specified site for some portion of its life span. Therefore, indigenous species can be useful as an indicator to assess environmental risk caused by hazardous chemicals in a specific site. So far a few toxicity studies using freshwater species which are indigenous to Korea have been carried out. In this study, a freshwater shrimp (Neocardina denticulata) indigenous to Korea was used for acute toxicity test of heavy metals. Neocardina denticulata were exposed to cadmium chloride (CdCl₂), copper chloride (CuCl₂) and zinc chloride (ZnCl₂) using automatic flow-through system for 96 hours. The 96h LC50s were calculated as 0.043 (0.042~0.045) ㎎ CdCl₂/L, 0.104 (0.098~0.113) ㎎ CuCl₂/L and 2.021 (1.633~2.594) ㎎ ZnCl₂/L. When compaired with some international standard species such as medaka (Oryzias latipes), Neocardina denticulata had high sensitivity. Therefore, this study suggested that Neocardina denticulata have possibilities for a sensitive test species to test heavy metal toxicity in aqua-system.