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Journal of Environmental Toxicology 2006;21(3):291-299.
항안드로겐성 물질이 성 성숙 이전 단계의 정소에서 미치는 영향 연구
홍진, 한순영, 문현주, 강태석, 강일현, 김태성, 김승희, 권기성
Early Exposure to Anti-androgen Compounds Induces the Delay in the Testis Development in Immature Male Rat
Jin Hong , Soon Young Han , Hyun Ju Moon , Tae Seok Kang , Il Hyun Kang , Tae Sung Kim , Seung Hee Kim , Ki-Sung Kwon
ABSTRACT
  The experiments investigated whether early exposure to testosterone propionate (TP) during prepuberty alters testis development in Sprague-Dawley male rats. We performed Hershberger assay using the stimulated weanling male rats by OECD protocols, cDNA microarray, and Western blot. TP was subcutaneously injected to uncastrated Sprague-Dawley male rat of 22 days old for 10 consecutive days at doses of 0.4, 0.8 ,1.0, 1.2, 1.6 mg/kg per day. At necropsy, the following tissues were removed and weighed: combined testes, epididymides (Epi), Cowper’s glands (COW), levator ani, and bulbocavernosus muscles (LABC), seminal vesicles, together with coagulating gland (SV) and ventral prostate (VP). We found that TP increased the weights of Epi, VP, SV, COW, and LABC, while testis was decreased in a dose-dependent manner. In cDNA microarray analysis of testis, there were significant reductions in the expression of cytochrome P450 11A (CYP11A), the rate-limiting enzyme of steroidogenesis.
  Taken together these results, TP exposure before puberty in male rats may produce the delay in testis development by inhibiting the CYP11A gene expression.
Keywords: OECD validation; Hershberger assay; TP; immature male rat
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