From the harmfulness expectation test conducted through a toxicity anticipation program, methylcyclohexane turned out to be harmful and simulative, but no carcinogenicity was anticipated. In a four-hour acute inhalation toxicity test, the result showed that lethal concentration (LC??) was 3,750 ppm (15,054 ㎎/L), which was identified as a harmful substance on the basis of the harmful substance classification standard 2<LC??≤20㎎<L)/4 hr (rat) of the Industrial safety and health law. methylcyclohexane fell under the category 4 (2,500<LC??≤5,000 ppm) substance from the GHS standard acute toxicity harmfulness classification. Also, from subchronic inhalation toxicity test that included 6 hours a day, five days a week, and for 13 weeks, we could observe weight, activity, long term weight, blood and blood biochemical influence from the exposure of test substance. No-observed effect level (NOEL) was determined below 100~400 ppm inboth male and female. This material falls under the Category 2 (50~250 ppm/6 hours/90 days) in the GHS (Globally Harmonized System) standard trace long-term whole body toxicity repeated exposure, and can be classified as a harmful substance in accordance with the Industrial Safety and Health Law harmful substance standard NOEL≤0.5 ㎎/L/6 hr/90 day (rat).