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Journal of Environmental Toxicology 2005;20(2):187-194.
Microarray를 이용한 Octachlorostyrene-노출 송사리 (Oryzias latipes)에서의 분자생물학적 지표연구
유대은, 강미선, 박은정, 김일찬, 이재성, 박광식
Molecular Biomarkers of Octachlorostyrene Exposure in Medaka, Oryzias latipes, using Microarray Technique
Dae-Eun You , Misun Kang , Eun-Jung Park , IL-Chan Kim , Jae-Seong Lee , Kwangsik Park
ABSTRACT
Octachlorostyrene (OCS) is a primarily concerning chemical in many countries because of its persistent and bioaccumulative properties in the environment. OCS is not commercially manufactured or used but it may be produced during incineration or chemical synthetic processes involving chlorinated compounds. There are several reports that OCS was found in the waters, sediments, fish, mussels, and also in human tissues. However, systematic studies on the OCS toxicities are scarce in literature. In this study, we tried to get the gene expression data using medaka DNA chip to identify biomarkers of OCS exposure. Medaka (Oryzias latipes) was exposed to OCS 1 ppm for 2 days and 10 days, respectively. Total RNA was extracted and purified by guanidine thiocyanate method and the Cy3- and Cy5-labelled cDNAs produced by reverse trancription of the RNA were hybridized to medaka microarray. As results, eighty five genes were found to be down-or upregulated by OCS. Some of the genes were listed and confirmed by real-time PCR.
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