Heave Metal Toxicity Test in Moina macrocopa with Glucose-6-Phsphate Dehydrogenase Activity

, Yong-seok Park; , Sang-Goo Lee; , Seung-Jin Lee; , Seung-Kyung Moon; , Eun-Joo Choi; , Ki-Tae Rhie

Published online January 1, 1986

Journal of Environmental Toxicology 2003;18(4):305-310.

Glucose-6-phosphate dehydrogenase를 이용한 Moina macrocopa의 중금속 독성 검정

박용석, 이상구, 이승진, 문성경, 최은주, 이기태

Heave Metal Toxicity Test in Moina macrocopa with Glucose-6-Phsphate Dehydrogenase Activity

Yong-seok Park , Sang-Goo Lee , Seung-Jin Lee , Seung-Kyung Moon , Eun-Joo Choi , Ki-Tae Rhie

ABSTRACT

A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metal toxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as an effective criterion in water quality. The toxicity of Pb (lead) and Cd (cadmium) for water flea, Moina macrocopa, were evaluated for 2~8 days with variables of mobilization ability. And the reproduction impairment of Moina macrocopa were investigated as the parameter of chronic toxicity test for Pb and Cd. As a result, the EC50 for immobilization of Moina macrocopa were Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproductive impairment to Moina macrocopa for Pb and Cd were 9.5938 and 8.3264 in EC50. A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopa was observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity of Moina macrocopa can be used as an indicative parameter in aquatic toxicity tests for heavy metals.