Isolation and Selection of Actinomycetes Producing Anti-fungal Materials

, Hyuk-Ku Kwon; , Byeong-Kon Kang; , Jang-Hoon Lee

Published online January 1, 1986

Journal of Environmental Toxicology 2003;18(2):131-136.

항진균성 활성물질을 생성하는 토양방선균의 분리

권혁구, 강병곤, 이장훈

Isolation and Selection of Actinomycetes Producing Anti-fungal Materials

Hyuk-Ku Kwon , Byeong-Kon Kang , Jang-Hoon Lee

ABSTRACT

Anti-fungal materials producing bacteria were isolated from soil hy bennett's agar and actinomycete isolation agar medium. The bacteria were identified as synonym of Actinomycetes. Based on the data obtained from its morphological and colony characteristics. The medium for production of anti-fungal materials was YEME (yeast extract 4g. malt extract 10g, glucose 4g. D.W 1l, pH 7.0±0.2). The culture conditions were 30℃, 7 days and 200 rpm in shaking incubator. No. 13, No. 15 and No. 28 strains were produced anti-fungal materials against fungal plant pathogens. Specially, The No. 28 strain showed a powerful biopesticide activity and broad spectrum effects of anti-fungal materials on Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.