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Korean Journal of Environmental Toxicology 2002;17(3):265-272.
효소 저해법을 이용한 유기인계 및 Carbamate계 농약의 다성분 잔류 검출
Detection for Multiresidue of the Organophosphorus and Carbamate Pesticides by Enzyme-Inhibition Method
Jung- Ho Kim
This study was carried out with the detection for multiresidue of the organophosphorus pesticides such as malathion, parathion, diazinon, and carbamate pesticide such as carbaryl, by enzyme-inhibition method. The acetylcholinesterase (AChE) and cholinesterase (ChE) activities in chicken braln determined by the Ellman's method were 166.6 and 5.8 μmol/min/g protein, and in chicken plasma were 23.1 and 8.3 μmol/min/g protein, respectively. The optimum pH of AChE and ChE was 8.2 and 7.8, respectively. The Km of AChE and ChE was 0.034 and 0.045 mM, respectively. I_(50) for AChE and ChE by some organophosphorus was 55.82 and 99.42 mg/L of malathion, 31.16 and 29.13 mg/L of parathion, and 17.89 and I9.62mg/L of diazinon, respectively. I_(50) for AChE and ChE by carbaryl of carbamate was 0.10 and 0.05 mg/L, respectively. The 0.07 mg/L of drinking water advisory level for carbaryl could be detected with I_(50) of AChE and ChE. Enzyme-Inhibition (EI) method with AChE and ChE was used the multiresidue method to detect the 1 mg/L of the carbamate pesticides.
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