Method Validation for the Simultaneous Analysis of Organophosphorous Pesticides in Blood by GC/MS

, Mee Jung Park; , Ja Youl Yang; , Ki Wook Kim; , Yoo Shin Park; , Hee Sun Chung; , Sang Ki Lee

Published online January 1, 1986

Journal of Environmental Toxicology 2005;20(4):297-302.

GC/MS 를 이용한 혈액 중 유기인제류 농약의 동시 분석에 관한 방법의 유효화

박미정, 양자열, 김기욱, 박유신, 정희선, 이상기

Method Validation for the Simultaneous Analysis of Organophosphorous Pesticides in Blood by GC/MS

Mee Jung Park , Ja Youl Yang , Ki Wook Kim , Yoo Shin Park , Hee Sun Chung , Sang Ki Lee

ABSTRACT

The purpose of this study was to provide the standard method for the analysis of organophosphorous pesticides such as chlorpyrifos, diazinon, malathion and parathion in blood. We performed method validation for these pesticides in blood according to EURACHEM(A focus For Analytical Chemistry in Europe) guide. For the analysis of the pesticides, we used solid-phase extraction column(Waters Oasis HLB®). After the extraction, the supernatants were evaporated to dryness under the nitrogen stream. They were analyzed by gas chromatography/mass spectrometry(GC/MS) after reconstituting with ethanol. Terbufos was used as an internal standard. To validate this method, we performed verification procedures with the following parameters : selectivity, linearity of calibration, accuracy, precision, limit of detection and quantification. Validation data according to Eurachem guide were adequate for our purpose fot the analysis of chlorpyrifos, diazinon, malathion and parathion in blood.